The Vizcarra group investigates how actin, the most abundant protein in many cells, is regulated to perform its impressive list of roles – from pushing crawling cells forward to separating cells when they divide. The focus will be on protein interactions that control the assembly and maintenance of actin rich stereocilia that are the “hairs” of inner ear hair cells. Her group will also work on engineering actin binding proteins with the goals of understanding their evolution and creating new, useful imaging probes and therapeutics.
BC3355, Biochemistry Laboratory Techniques
BC3283, Biochemistry II
Related to current research:
Metavinculin tunes the flexibility and the architecture of vinculin-induced bundles of actin filaments. Oztug Durer ZA, McGillivary RM, Kang H, Elam WA, Vizcarra CL, Hanein D, De La Cruz EM, Reisler E, Quinlan ME, J. Mol. Biol. 2015 427: 2782–2798.
The role of formin tails in actin nucleation, processive elongation, and filament bundling. Vizcarra CL, Bor B, and Quinlan ME, J. Biol. Chem. 2014 289: 30602–30613.
Interaction between microtubules and the Drosophila formin Cappuccino and its effect on actin assembly. Roth-Johnson EA, Vizcarra CL, Bois JS, and Quinlan ME, J. Biol. Chem. 2013, 289: 4395–4404.
Autoinhibition of the formin Cappuccino in the absence of canonical autoinhibitory domains. Bor B, Vizcarra CL, Phillips ML, and Quinlan ME, Mol. Biol. Cell 2012 23: 3801–3813.
Structure and function of the interacting domains of Spire and Fmn-family formins. Vizcarra CL*, Kreutz B*, Rodal AA, Toms AV, Lu J, Zheng W, Quinlan ME, and Eck MJ, Proc. Natl. Acad. Sci. USA 2011 108: 11884–11889. *equal contribution
Comparison of random mutagenesis and semi-rational designed libraries for improved cytochrome P450 BM3- catalyzed hydroxylation of small alkanes. Chen MM, Snow CD, Vizcarra CL, Mayo SL, and Arnold FH, Protein Eng. Des. Sel. 2012 25: 171–178.
An improved pairwise decomposable finite difference Poisson-Boltzmann method for computational protein design. Vizcarra CL, Zhang N, Marshall SA, Wingreen N, Zeng C, and Mayo SL, J. Comp. Chem. 2008 29: 1153–1162.
Computationally designed libraries of fluorescent proteins evaluated by preservation and diversity of function. Treynor TP, Vizcarra CL, Nedelcu D, and Mayo SL (2007). Proc Natl Acad Sci USA 2007 104: 48–53.
One- and two-body decomposable Poisson- Boltzmann methods for protein design calculations. Marshall SA, Vizcarra CL, and Mayo SL, Protein Sci 2005 14: 1293–1304.
Electrostatics in Computational Protein Design. Vizcarra CL and Mayo SL, Curr. Opin. Chem. Biol. 2005 9: 622–629.